Blood-typing of economically important species such as cattle, horses, pigs and sheep is carried out extensively throughout the world, primarily for the purpose of parentage identification for pedigree registration. Most bloodtyping reagents are prepared by alloimmunization of animals with appropriately chosen red blood cells. The antisera thus produced usually have to be selectively absorbed with other red cells until all unwanted specificities have been removed. Although, often termed 'monospecific', such alloantibodies are in fact very heterogeneous and there is no guarantee that repeated bleedings from the same recipient animal will yield identical antibodies. Reagents prepared in this way have to be subjected to rigorous standardization tests before they can be used as internationally accepted blood-typing reagents. It is also necessary for laboratories that produce blood-typing reagents to maintain a permanent panel of animals to act as donors and recipients and to provide red cells embracing all known antigenic specificities. All the above-mentioned procedures are time- consuming and expensive, and it is not surprising therefore that, since the advent of the hybridoma technique (KBhler 5 Milstein, 1975) hopes have been raised that monoclonal antibodies produced in culture might provide a satisfactory alternative to conventionally-produced blood-typing reagents.

E. M Tucker, L.J. Wright

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 6. Round tables, , 359-364, 1982
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