One-cell ova were recovered from superovulated rabbit and pig donors. For microinjection a DNA fragment consisting of the structural gene of the human growth hormone and the promotor region of the mouse methallothionein gene was used.
After transfer of injected eggs 37 (9.6 %) offspring were born in rabbits and 15 (3.4 %) in pigs. 5 rabbits and 1 piglet were found to be transgenic by dot hybridisation and Southern blotting.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XII. Biotechnology, selection experiments, parameter estimation, design of breeding systems, management of genetic resources., , 45–50, 1986
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