We have described conditions which are sufficient for the establishment of stable, pluripotent cell lines from porcine and ovine blastocysts. The general morphologies and growth characteristics of the porcine and ovine cells in culture are very similar. Furthermore, the porcine and ovine cells bear close resemblance to cells in primary outgrowths from inner cell masses, and display features which are characteristic of murine embryonic stem cells: namely, large, translucent nuclei with many prominent nucleoli, and relatively sparse cytoplasm. These observations, together with the capacity of the cells to differentiate spontaneously in culture into a range of discernible phenotypes, support the contention that the porcine and ovine cell lines represent primary ectodermal lineages. Differences in the rates and patterns of growth of ungulate compared with murine embryonic stem cells suggest that the identification and isolation of pluripotential cell lines is the same for ungulate, but different for murine species.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIII. Plenary lectures, molecular genetics and mapping, selection, prediction and estimation., , 58–65, 1990
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