We have designed a rapid test, based on the polymerase chain reaction (PCR) and allele-specific oligonucleotides to ascertain the presence of two alleles (A 3 and B) at the B-casein locus. Blood and semen can be used as sources of D NA for the test. Survey o f 108 Holstein-Friesian bulls confirms that both these alleles are rare in the population and that the B allele is tightly linked to the B allele of K-casein.

D. Savva, S. J Pinder, C. J Skidmore

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIV. Dairy cattle genetics and breeding, adaptation and conservation., , 245–247, 1990
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