Abstract

A cDNA coding for ornithine decarboxylase was isolated from a bovine liver cDNA library. The clone (1760 bp) consisted of 5’- and 3’-untranslated regions of 187 nucleotides each and an open reading frame of 1383 nucleotides encoding an ODC protein (Mr. 51,342 daltons) of 461 amino acids. Comparison of the nucleotide and the predicted amino acid of the cDNA to other mammalian ODCs showed a very high degree of homology both at the DNA and protein levels. The bovine ODC mRNA was identified by Northern blot to be a single species with a molecular size of 2.35 kb. Primer extension analysis indicated that the 5’-untranslated region of the bovine ODC mRNA was 314 nucleotides long. Southern blot analysis of bovine genomic DNA revealed restriction fragment length polymorphisms when cleaved with restriction enzymes PstI, MspI, TaqI and Bgll.

J. Yao, D. Zadworny, U. Kuhnlein, J. F Hayes

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 129–132, 1994
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