Eighteen pairs of female twins were genotyped for 3 to 9 microsatellites using diplex PCR systems from blood and hair roots. Blood chimaerism was evident in 17 out of 18 sets of twins tested. Chimaerism was recognized by discrepancies between blood and hair analyses for microsatellite markers and by detection of more than 2 alleles per genotype. Chimaerism of 2, 3 and 4 alleles were shown in genotypes of twins from blood as compared to 1 and 2 alleles from hair. We found that genotyping of microsatellites from hair follicles is a highly sensitive method for discrimination between the inherited and acquired genotypes in dizygotic twins.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 105–108, 1994
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