Ova collected from diploid female trout were inseminated with sperm from triploid males, diploid males or with a mixture of both (2nX3n, 2nX2n and 2nX2n + 3n crosses). Survival from fertilization to hatching was 0.57% in 2nX3n crosses, 10.7% in 2nX2n crosses, and 10.9% in 2nX2n + 3n crosses, and it was affected by low egg quality. Survival from hatching to 4 months was 10%, 65% and 44% for the 2nX3n, 2nX2n and 2nX2n + 3n crosses (P< 0.05). Hatching embryos in 2nX3n crosses exhibited morphological abnormalities, although a few juveniles were obtained and one of them was a sexually maturing precocious male. Ploidy of parents and offspring was examined by flow cytometry and by image analysis of modified Azure A-stained tissue smears. Flow cytometry on red blood cells and image analysis of erythrocytes and hepatocytes revealed a hypertriploid genome in all 2nX3n offspring and in 12.5% of 2nX2n + 3n progeny. Metaphase plates analyzed in gill epithelia from these individuals revealed aneuploid figures and multiple levels of ploidy. These data provide the first evidence of limited functional fertility of triploid trout males.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 19. Selection and quantitative genetics; growth; reproduction; lactation; fish; fiber; meat., , 395–397, 1994
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