A bovine genomic library was screened for the presence of AGCn repeats. 35 positive clones were isolated. 17 were sequenced and found to include between 5 and 9 AGC repeats. All AGC repeats were located adjacent to the 3' end of bovine SINE elements. PCR reactions using either two unique primers or one unique and one SINE primer produced high resolution products without the secondary artifact ladders typical of dinucleotide microsatellites. 6 AGC microsatellites were found to be polymorphic with 2-4 alleles each and PIC values ranging between 0.26 and 0.49. One microsatellite, AR025, was mapped to chromosome 26 with the CSIRO reference families. Because of their strong association with AGC repeats and high frequency in the genome SINE-31 PCR may prove to be a novel source of polymorphic trinucleotide markers in the bovine genome
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 125–128, 1994
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