In order to anchor the linkage map to the physical map we constructed a porcine cosmid library. A total of 2400 clones were picked and gridded and subsequently screened by a [GT]io oligonucleotide yielding a total of 850 GT-positive clones. The chromosomal localization of 103 [GT]n-contammg clones as well as three cosmids showing VNTR polymorphism were determined by fluorescence in situ hybridization. A total of 86 [GT]n-containing cosmids and the three VNTRs showed a unique hybridization site. Cosmids mapped by fluorescence in situ hybridization and shown to be m areas of interest was sequenced to determine the sequences flanking the microsatellite in order to make PCR- primers. Genotyping was performed in the reference families established for the European effort to maks genetic maps of the porcine genome (PiGMaP).
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 68–70, 1994
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