Somatostatin is physically mapped to bovine chromosome 1q23-q25 by in situ hybridization. A genomic somatostatin probe was isolated from a bovine 1;29 library, prepared previously in our lab, with human somatostatin genomic DNA. Chromosomes were prepared from blood taken from a Charolais bull (59,XY 1;29,tt). The probe was radiolabelled with tritium for in situ hybridization. After autoradiography the slides were developed and chromosomes Giemsa-stained and examined for points of hybridization. A histogram was constructed, recording the number and position of dark grains on the 1,29t chromosomes. A peak was found to occur at chromosome 1 q23-q25. The results were statistically analyzed with a Zmax test and were found to be significant at the 5% level. This is the second type I locus to be mapped to the center of chromosome 1, providing a valuable marker for this chromosome, and fulfilling the goal for three well-spaced markers on each bovine chromosome. It also provides further evidence for sequence homology between bovine chromosome 1 and the q arm of human chromosome 3.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 60–62, 1994
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