The Booroola (FecB) locus has been mapped to sheep chromosome 6. M arkers flanking this gene were identified in sheep x ham ster somatic cell hybrids containing chromosomes 6 and 24. Partial genomic libraries were constructed from the cell lines, sheep-specific clones were selected from the libraries and microsatellite markers developed. Four markers mapped to sheep chromosome 6 and one marker mapped to sheep chromosome 24, demonstrating that chromosome-specific clones were generated from the hybrid cell lines and further defining the position of the FecB locus on sheep chromosome 6.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 17–20, 1994
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