The objective of this study was to analyse the secretion of embryonic interferon (oTP) by ovine conceptus and to relate it with embryo survival of its dam. Two groups of Romanov ewes were selected for their high (H - n=32) or low (L - n = 33) breeding value for embryo survival. Intra-uterine insemination were done; at day 10, 12 and 15 of pregnancy, uterine horns were flushed with PBS and embryos were incubated for 24 hours. oTP was assayed by radio-immunoassay in flushing and in medium culture. Total oTP content in flushings increased widely from day 10 (p= 1.8 ng ; se=0.4), to day 12 (u=3.4 pg ; se=1.5) and day 15 (p=72.8 pg ; se=6.1) of pregnancy, it was more related to the age than to the embryo size : that shows the very fast change of activity of the trophoblast cells. The H group presented more total oTP than the L group, the difference was significant on day 10 and vanished by day 15. On day 15 of pregnancy all flushings got similar amount of oTP suggesting the existence of a global regulation at the beginning of implantation. In vitro, the H embryos secreted also higher amount of oTP. On day 10 of pregnancy that was not explained by the size difference of the embryos. Variance analysis showed significant group and dam effects on oTP secreted by the Day 10 embryos, which could mean that maternal effect plays a role in the early development of the embryo and in its ability to secrete oTP.

Loys Bodin, P. Reinaud, D. Lajous, J. P Poivey, G. Charpigny

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 19. Selection and quantitative genetics; growth; reproduction; lactation; fish; fiber; meat., , 245–248, 1994
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