The high growth (hg) locus is a major locus that increases weight gain and mature body size in mice A previous mapping study positioned hg to an interval (hg candidate interval) on mouse chromosome 10. To saturate this interval with more markers, we utilized the random amplified polymorphic DNA (RAPD) assay to analyze two DNA pools created from F2 mice. The pools differed for the alleles in the hg candidate interval, while being genetically similar outside this interval. Screening of 888 RAPD primers identified eight markers that map to the hg candidate interval. These RAPD markers will be used for fine genetic mapping of hg, and will facilitate future map-based cloning of this locus. The approach used in this study was successful in targeting markers to a specific genomic interval in mice, and should be also applicable in domestic animal species.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 71–74, 1994
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