Cross-bred cattle are produced in India to increase the frequencies of desired k-casein -B (CASK-B) allele as well as (3-casein A2 (CASB-A2) and B (CASB-B) alleles in them because of economic importance of these alleles in industrial milk production. To ascertain that the required goals were achieved, frequencies of the alleles of CASK and CASB in Cross-breds and Sahiwal breed of cattle were studied using Polymerase Chain Reaction (PCR) and Sequence Specific Oligonucleotide Probes (SSOP). The results showed predominance of CASK-A allele in Sahiwal while CASK-B allele was predominant in Cross-breds. At CASB level CASB-A2 allele showed predominance in both Sahiwal and Cross-breds and an increased frequency of CASB-B allele in Crossbreds.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 26: Quantitative genetic theory; selection theory and experiments; internationalisation of breeding programs; detection of quant, , 381–384, 1998
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