In broilers, excessive adiposity is a major drawback for production, reducing feed efficiency and lean meat yield (Leclercq, 1988 ; Griffin et al., 1992). In order to decipher the mechanisms involved in the regulation of fatness in the chicken, lean and fat chicken lines have been divergently selected for adipose tissue weight (Leclercq et al., 1980). Some studies performed in these lines have suggested a higher lipogenesis rate in the liver of fat chickens. Thus, slight differential expressions were found for some few genes directly related to lipid synthesis and secretion (Daval et al., 2000). In order to analyze the expression of more genes, differential display analyses (Liang and Pardee, 1992) were performed on RNAs extracted from lean and fat chickens. Differential displayed products were then used as radiolabelled probes for northern-blot analyses and confirmation of differential expression. 

E. Bourneuf, W. Carre, M. Douaire, C. Diot

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 2002. Session 11, , 11.18, 2002
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