To examine the effects of diet supplementation on the expression of genes related to lipid metabolism, 28 Angus steers were fed either a pasture only, pasture with soyabean and corn oil, pasture with corn grain, or high concentrate diet. At slaughter samples of s.c. adipose tissue were collected from which RNA was extracted. Relative abundance was measured using the Affymetrix Bovine array. An ANOVA model nested within gene was used to analyze the background adjusted, normalized average difference of probe-level intensities. To control experiment wise error, a false discovery rate of 0.05 was imposed on all contrasts. Results show that changes in diet may significantly alter the expression of genes associated with fatty acid metabolism, such as stearoyl-CoA desaturase and Fatty acid synthase.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume , , 23.18, 2006
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