Severe Combined Immunodeficiency Disease (SCID) of horses was appeared as a deficiency of T and B lymphocytes in foals and resulted in death, caused by infection with opportunistic pathogens, by 3 month of age. This disease is a genetic autosomal recessive trait. A 5-basepair deletion in the horse gene encoding DNA-protein kinase catalytic subunit (DNA-PKCS) was discovered which is responsible for SCID in Arabian horses. We have developed a PCR-based test for detection of the gene defect base on 5-basepair deletion in DNA-PKcs gene using allele-specific primers for amplification of DNA containing the 5-basepair deletion. (a region by 259bp). The carriers are heterozygote (259 and 254 bp bands) and others are homozygote (259bp). We tested 120 Iranian Arab horse. However, the results showed all samples were homozygous at 259 bp i.e. noncarriers. In conclusion, we suggest further study on Arab horses throughout of Iran to identify any carriers.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume , , 22.38, 2006
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