Abstract

{SUMMARY: Recent work using 16s rRNA sequencing has led to the understanding that milk microbiota is complex, harboring great multifunctional diversity. An important question is if milk in the mammary gland is sterile and bacteria come from the external environment or if there are endogenous bacteria present in milk. In the present study, we performed a preliminary metagenome analysis of bovine milk by sequencing the V1–V2 hypervariable regions of 16S rRNA gene. Milk samples were collected from the same cow at 3 different stages of lactation (15, 90, 120 dim), with and without using a cannula. Microbial DNA was extracted from two tissues, somatic cells and fat. Our findings showed: 1) The microbiota structure of the two tissues was completely different (adonis R2 = 0.68

Juan F Medrano, Anna Cusco, AIma Islas-Trejo, Armand Sanchez, Olga Francino

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology & Species - Bovine (dairy) 2, , 343, 2018
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