Porcine reproductive and respiratory syndrome virus (PRRSV) is a major respiratory pathogen of swine that has become extremely costly to the swine industry worldwide, often causing losses in production and animal life due to their ease of spread. However, the intracellular changes that occur in pigs following viral respiratory infections are still scantily understood for PRRSV, as well as, other viral respiratory infections. The aim of this study was to acquire a better understanding of PRRS disease by comparing gene expression changes that occur in tracheobronchial lymph nodes of pigs infected with either porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), or swine influenza virus (IAV) infections. The study identified and compared gene expression changes in the TBLN of pigs infected with either PRRSV, PCV2, IAV, or sham inoculation.  Five pigs from each group were euthanized on 1, 3, 6, and 14 DPI.  TBLN was collected from each pig and total RNA was pooled for each group at each time-point to make 16 libraries for analysis by Digital Gene Expression Tag Profiling (DGETP). The data underwent standard filtering to generate a list of sequence tag raw counts that were then analyzed using multidimensional and differential expression statistical tests. The results showed that PRRSV, IAV and PCV-2 viral infections followed a clinical course in the pigs typical of experimental infection of young pigs with these viruses. Gene expression results echoed this course, as well as, uncovered genes related to shared and unique host immune responses to the 3 viruses. By testing and observing the host response to other respiratory viruses, our study has elucidated similarities and differences that can assist in development of vaccines and therapeutics that shorten or prevent a chronic PRRSV infection. Keywords: PRRSV, PCV-2, IAV, lymph node, gene expression, functional genomics

Laura Miller, Damarius Fleming, Greg Harhay, Marcus Kehrli, Kelly Lager

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology - Disease Resistance 2, , 672, 2018
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