Deciphering the role of vaginal and sperm metagenome in the success of artificial insemination in sheep M.M. Serrano1, F. Freire2, J.F. Martínez-Blanch3, C. González1, L. Reyes2, M.C. Solaz-Fuster3, J.H. Calvo4,E. Climent3, M.A. Jiménez1 & F.M. Codoñer3 1INIA Dpto. Mejora Genética Animal, Ctra. de la Coruña km 7.5, 28040 Madrid, Spain 2OVIGEN, Granja Florencia s/n, 49800 Toro, Zamora, Spain 3Lifesequencing SL, Parc Científic Universitat de València, 46980 Paterna, Valencia, Spain 4Unidad de Tecnología en Producción Animal CITA, 59059 Zaragoza, Spain. ARAID, 50004 Zaragoza, Spain fcodoner@lifesequencing.com (Corresponding Author) For the first time the vaginal and sperm microbiota of ewes and rams relative to artificial insemination (AI) success have been studied. Hypervariable regions V3 and V4 of the 16s ribosomal gene have been sequenced with a MiSeq Illumina platform in 50 ewes from 5 different herds and 5 AI rams. Microbiota differences at the phylum, family, genera and species were tested for antibiotic treatment in progesterone releasing intravaginal devices (PRIDs), pregnancy rate, AI ram and herd. Results showed that antibiotic treatment only affects the presence of Streptobacillus which was 21 times more abundant (padj=0.0002) in ewes carrying PRIDs without antibiotic. One species of this genus, Streptobacillus notomytis, was 19 times more abundant in non-pregnant that in pregnant ewes, which reveals the efficiency of framycetin in the PRIDs to increase AI fertility. Mageebacillus, Histophilus, Actinobacilllus and Sneathia genera were 70, 25, 22 and 13 (p_adjusted<0.05) times more abundant in non-pregnant than in pregnant ewes. These genera are also highly overrepresented in two of the three herds with the lowest AI success. Mageeibacillus genus belongs to the family Ruminococcaceae and has been recovered from the genital tract of women with bacterial vaginitis. Sneathia has been associated with bacterial vaginosis, spontaneous abortion and other invasive infections in woman. Actinobacillus and Histophilus belong to the HAP group (Haemophilus-Actinobacillus-Pasteurella), which includes great number pathogens resistant to most cellular defence mechanisms, and are related with epididymitis, vaginitis, placentitis, infertility, abortions in the ovine species. However, Actinobacillus and Histophilus were not detected from the sperm samples of the AI rams here analysed. The high presence of these genera in non-pregnant ewes and herds with low AI success could be due to the venereal transmission of these microorganisms by natural mating with rams exiting in the herds. Keywords: metagenomics, fertility, reproductive tract, sheep

Magdalena Serrano, Fernando Freire, Juan Martínez-Blanch, Carmen González, Luis Reyes, Carmen Solaz-Fuster, Jorge Calvo, Eric Climent, Angeles Jiménez, Francisco Codoñer

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology - Reproduction 1, , 106, 2018
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