Abstract

Identification of genes affecting intramuscular fat content in Iberian pigs through Longissimus dorsi transcriptomic analyses. Iberian pigs have a high content of intramuscular fat (%IMF) which explains the remarked quality of its meat and dry-cured products. The discovery of genes that codify for proteins involved in intramuscular fat content is very valuable for searching polymorphisms useful in a selection scheme to maintain or improve this trait. The objective of this study was to analyse through RNAseq technique differentially expressed (DE) genes on Longissimus dorsii in two groups of a selected Iberian line with extreme breeding values for IMF. A total of 221 and 116 DE genes and potentially new isoforms, respectively, were detected. The Gene Ontology enrichment analyses and Genome-Scale Metabolic Network (Recon) showed an enrichment of DE genes in pathways related with skeletal muscle development, fatty acid metabolism and adipogenesis. In addition to this, the analyses with VarElect showed candidates genes for IMF previously reported as SCD and other no previously studied as EGR1, PFKFB3 or ARID5B among others. These genes are very promising as candidate genes for intramuscular fat, the detection of polymorphisms and their inclusion in the breeding scheme will be evaluated in a close future. Keywords: Iberian pigs, intramuscular fat, meat quality, transcriptome

Maria Muñoz, Juan María García-Casco, Fernando Sánchez-Esquiliche, Fabián García, Miguel Ángel Fernández-Barroso, José María Pariente, Fernando Gómez, María del Carmen Rodríguez, Luis Silió

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Electronic Poster Session - Molecular Genetics 4, , 412, 2018
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