To elucidate the crucial interaction between porcine reproductive and respiratory syndrome virus (PRRSV) and bacterial lipopolysaccharides (LPS) in the development of host immunity, the present study investigated the effect of in vivo PRRSV vaccination on LPS-induced cytokine mRNA expression patterns in the peripheral blood mononuclear cells (PBMCs). The PBMCs were isolated from the whole blood samples collected at before vaccination (D0), and day 1 (D1), 14 (D14) and day 28 (D28) post primary PRRSV vaccination in Pietrain pigs. The PBMCs collected at each time point were then stimulated with LPS (@ 0.5 ug/ml) for 24 h in vitro. The relative mRNA expressions of IFNγ, TNFα, IL1β, IL10, TLR4, NFkB, IRF3 and MyD88 were quantified by qRT-PCR followed by protein measurement of IL-1β and TNF-α in the cell culture supernatants using by ELISA. PRRSV vaccination resulted in upregulation of IL1β and MyD88 while LPS stimulation increased the TLR4 expression in the naïve PBMCs. Although, PRRSV vaccination induced an upward trend of TNFα, IFNγ, IL1β, TRL4 and NFkB expression, subsequent in vitro LPS stimulation to the vaccine-primed PBMCs resulted in more pronounced expression of these cytokines. We concluded that the PRRSV vaccination in pigs is permissive to PBMCs and may sensitize them in such way that enhances the cytokine mRNA expression upon subsequent exposure to LPS. Keywords: PRRSV-LPS interaction, innate immunity, PBMCs, cytokines, pig
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Electronic Poster Session - Biology - Disease Resistance 2, , 605, 2018
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