Rate of de novo mutation in dairy cattle and potential impact of reproductive technologies C. Harland1,4, K. Durkin4, M. Artesi4, L. Karim2, N. Cambisano2, M. Deckers2, N. Tamma2, E. Mullaart3, W. Coppieters2, C. Charlier4 & M. Georges4 1Livestock Improvement Corporation, New Zealand; 2GIGA Genomics platform, University of Liege, Belgium; 3CRV, Arnhem, The Netherlands; 4Unit of Animal Genomics, GIGA-R & Faculty of Veterinary Medicine, University of Liège, Belgium To study the process of de novo mutation (dnm) in the bovine germ-line, we have sequenced the whole genomes of 743 dairy cattle constituting 131 sire-dam-offspring trios with an average of five grand-offspring each. A first study using five families revealed the common occurrence of somatic and germ-line mosaicism for dnms, pointing towards mutation-prone early cleavage cell divisions. In this extended study, of 131 trios we have identified 7,498 dnms with an overall transition-transversion rate of 1.96, of which 3,413 are mosaic in either the proband or parents’ germ-line, confirming the results of our previous study. We detect a significant environmental effect resulting from the use of reproductive technologies, such as in vitro fertilisation, on the rate of dnm in the early embryo.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology - Reproduction 2, , 983, 2018
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